Chemotyping and Determination of Antimicrobial, Insecticidal, and Cytotoxic Properties of Wild-Grown Cannabis sativa from Nepal
- Prabodh Satyal (University of Alabama in Huntsville)
- William N. Setzer (University of Alabama - Huntsville)
Abstract
Cannabis sativa was collected from a wildgrowing population in Biratnagar, Nepal. The essential oil was obtained by hydrodistillation and analyzed by gas chromatography – mass spectrometry. A total of 107 constituents were identified in the oil accounting for 94.2% of the composition. This Nepalese chemotype is characterized by a predominance of sesquiterpenoids (68.1%) dominated by (E)-caryophyllene (20.4%), α-humulene (7.0%), and α-bisabolol (5.8%), but a paucity of monoterpene hydrocarbons (0.9%). In particular, neither myrcene nor terpinolene were detected. The oil in the Nepalese Cannabis plants did contain small amounts of cannabidiol (1.6%), cannabichromene (0.2%) and Δ9-tetrahydrocannabinol (0.4%). The essential oil from Nepalese Cannabis sativa was screened for antimicrobial, cytotoxic, larvicidal, and insecticidal activity, and it appears as though C. sativa is relatively non-toxic.
Keywords: Caryophyllene, cannabidiol, Cannabis indica, cluster analysis, humulene, tetrahydrocannabinol.
How to Cite:
Satyal, P. & Setzer, W. N., (2014) “Chemotyping and Determination of Antimicrobial, Insecticidal, and Cytotoxic Properties of Wild-Grown Cannabis sativa from Nepal”, Journal of Medicinally Active Plants 3(1-4), 9-16. doi: https://doi.org/10.7275/R58W3B8V
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